[Experimental study on A549 cell death mediated by xenoantigen α-gal 
in human serum].

ABSTRACT:

Background and objective

The absence of α-gal in humans is caused by the inactivity of α-1,3GT gene. However, humans have pre-existing and abundant anti-gal antibodies. Xenotransplantation procedures have indicated the high potential of introducing α-1,3GT gene to synthesize α-gal for cancer gene therapy by mimicking hyper-acute rejection. The aim of this study is to construct a lung cancer A549 cell line that expressed α-gal, and to observe the antitumor mechanisms mediated by human serum.

Methods

A549 cells were transfected with pEGFP-N1-GT plasmids constructed in a previous study. A stable transgenic cell line, A549-GT, was then selected and cultivated. The biological characteristics of A549-GT cells, including morphology and proliferation, were examined. α-1,3GT mRNA exp

SUBMITTER: Zhu S 

PROVIDER: S-EPMC6000037 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature