[Construction of A eukaryotic expression vector carrying the iNOS gene and its effect on A549 lung cancer cells].

ABSTRACT:

Background and objective

The iNOS gene is associated with NO-mediated antitumor effects. The aims of this study are to construct a eukaryotic expression plasmid that carries the iNOS gene and to detect the expression levels and antitumor effects of the iNOS gene on A549 lung cancer cells.

Methods

A DNA fragment of the human iNOS coding sequence was amplified using reverse transcription polymerase chain reaction (RT-PCR). The DNA fragment was subsequently cloned into the multiple cloning sites of the eukaryotic expression vector pVAX. The recombinant plasmid was confirmed using restriction enzyme treatment, PCR, and sequencing and was then transfected into A549 lung cancer cells. The expression of the iNOS gene in the A549 lung cancer cells after transfection was verified by

SUBMITTER: Ye S 

PROVIDER: S-EPMC6000129 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature