[Identification analysis of eukaryotic expression plasmid Rap2a and its effect on the migration of lung cancer cells].

ABSTRACT:

Background

Rap2a, a member of the small GTPase superfamily, plays a critical role in regulating the function of integrin and cell adhesion, thereby controlling cell motility and cell/matrix interactions. However, the function of Rap2a in carcinogenesis is still poorly understood. To clone Rap2a cDNA, which belongs to human Ras-related small G protein superfamily, we constructed its eukaryotic expression vector and determined its expression in lung cancer cells. The aim of this study is to explore the role of Rap2a in carcinogenesis.

Methods

The levels of endogenous Rap2a protein in lung cancer cells were measured by Western blot. Total RNA of human osteosarcoma cells U2OS was extracted and reverse-transcribed into cDNA by RT-PCR. Then, Rap2a gene was amplified by PCR and ins

SUBMITTER: Wu J 

PROVIDER: S-EPMC6000507 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature