[Interference of homologous sequences on the SNP study of CYP2A13 gene].

ABSTRACT:

Background and objective

It has been proven that cytochrome P450 enzyme 2A13 (CYP2A13) played an important role in the association between single nucleotide polymorphisms (SNP) and human diseases. Cytochrome P450 enzymes are a group of isoenzymes, whose sequence homology may interfere with the study for SNP. The aim of this study is to explore the interference on the SNP study of CYP2A13 caused by homologous sequences.

Methods

Taqman probe was applied to detect distribution of rs8192789 sites in 573 subjects, and BLAST method was used to analyze the amplified sequences. Partial sequences of CYP2A13 were emplified by PCR from 60 cases. The emplified sequences were TA cloned and sequenced.

Results

For rs8192789 loci in 573 cases, only 3 cases were TT, while the rest wer

SUBMITTER: Hua F 

PROVIDER: S-EPMC6000522 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature