Evaluation of buccal swabs for pharmacogenetics.

ABSTRACT:

Objective

A simple, non-invasive sample collection method is key for the integration of pharmacogenetics into clinical practice. The aim of this study was to gain samples for pharmacogenetic testing and evaluate the variation between dry-flocked and sponge-tipped buccal swabs in yield and quality of DNA isolated.

Results

Thirty-one participants collected samples using dry-flocked swabs and sponge-tipped swabs. Samples were assessed for DNA yield, quality and genotyping performance on a qPCR OpenArray platform of 28 pharmacogenetic SNPs and a CYP2D6 TaqMan copy number variant. DNA from sponge-tipped swabs had a significantly greater yield compared to DNA collected with dry-flocked swabs (p = 4.4 × 10^-7). Moreover, highest genotyping call rates across all assays and

SUBMITTER: Ang JS 

PROVIDER: S-EPMC6000964 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature