Genetic polymorphism in Leishmania infantum isolates from human and animals determined by nagt PCR-RFLP.

ABSTRACT:

Background

Leishmania infantum is the causative agent of human visceral leishmaniasis (VL) and sporadic human cutaneous leishmaniasis (CL) in the Mediterranean region. The genetic variation of the Leishmania parasites may result in different phenotypes that can be associated with the geographical distribution and diversity of the clinical manifestations. The main objective of this study was to explore the genetic polymorphism in L. infantum isolates from human and animal hosts in different regions of Morocco.

Methods

The intraspecific genetic variability of 40 Moroccan L. infantum MON-1 strains isolated from patients with VL (n = 31) and CL (n = 2) and from dogs (n = 7) was evaluated by PCR-RFLP of nagt, a single-copy gene encoding N-acetylglucosamine-1-phosphate transferase

SUBMITTER: El Hamouchi A 

PROVIDER: S-EPMC6001066 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature