E2F1 promotes hepatic gluconeogenesis and contributes to hyperglycemia during diabetes.

ABSTRACT:

Objective

Aberrant hepatic glucose production contributes to the development of hyperglycemia and is a hallmark of type 2 diabetes. In a recent study, we showed that the transcription factor E2F1, a component of the cell cycle machinery, contributes to hepatic steatosis through the transcriptional regulation of key lipogenic enzymes. Here, we investigate if E2F1 contributes to hyperglycemia by regulating hepatic gluconeogenesis.

Methods

We use different genetic models to investigate if E2F1 regulates gluconeogenesis in primary hepatocytes and in vivo. We study the impact of depleting E2F1 or inhibiting E2F1 activity in diabetic mouse models to evaluate if this transcription factor contributes to hyperglycemia during insulin resistance. We analyze E2F1 mRNA levels in the live

SUBMITTER: Giralt A 

PROVIDER: S-EPMC6001358 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature