Hyperpolarized ketone body metabolism in the rat heart.

ABSTRACT: The aim of this work was to investigate the use of ¹³ C-labelled acetoacetate and β-hydroxybutyrate as novel hyperpolarized substrates in the study of cardiac metabolism. [1-¹³ C]Acetoacetate was synthesized by catalysed hydrolysis, and both it and [1-¹³ C]β-hydroxybutyrate were hyperpolarized by dissolution dynamic nuclear polarization (DNP). Their metabolism was studied in isolated, perfused rat hearts. Hyperpolarized [1-¹³ C]acetoacetate metabolism was also studied in the in vivo rat heart in the fed and fasted states. Hyperpolarization of [1-¹³ C]acetoacetate and [1-¹³ C]β-hydroxybutyrate provided liquid state polarizations of 8 ± 2% and 3 ± 1%, respectively. The hyperpolarized T₁ values for the two substrates

SUBMITTER: Miller JJ 

PROVIDER: S-EPMC6001529 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature