Investigating capillary electrophoresis-mass spectrometry for the analysis of common post-translational modifications.

ABSTRACT: Capillary electrophoresis coupled to mass spectrometry is a very efficient analytical method for the analysis of post-translational modifications because of its high separation efficiency and high detection sensitivity. Here we applied CE-MS using three differently coated separation capillaries for in-depth analysis of a set of 70 synthetic post-translationally modified peptides (including phosphorylation, acetylation, methylation, and nitration). We evaluated the results in terms of peptide detection and separation characteristics and found that the use of a neutrally coated capillary resulted in highest overall signal intensity of singly modified peptides. In contrast, the use of a bare-fused silica capillary was superior in the identification of multi-phosphorylated peptides (12 out of

SUBMITTER: Faserl K 

PROVIDER: S-EPMC6001557 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature