Phosphate-dependent FGF23 secretion is modulated by PiT2/Slc20a2.

ABSTRACT:

Objective

The canonical role of the bone-derived fibroblast growth factor 23 (Fgf23) is to regulate the serum inorganic phosphate (Pi) level. As part of a feedback loop, serum Pi levels control Fgf23 secretion through undefined mechanisms. We recently showed in vitro that the two high-affinity Na⁺-Pi co-transporters PiT1/Slc20a1 and PiT2/Slc20a2 were required for mediating Pi-dependent signaling. Here, we addressed the contribution of PiT1 and PiT2 to the regulation of Fgf23 secretion.

Methods

To this aim, we used PiT2 KO and DMP1Cre; PiT1^lox/lox fed Pi-modified diets, as well as ex vivo isolated long bone shafts. Fgf23 secretion and expression of Pi homeostasis-related genes were assessed.

Results

In vivo, PiT2 KO mice responded inappropriately

SUBMITTER: Bon N 

PROVIDER: S-EPMC6001877 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature