Regional differences in brain glucose metabolism determined by imaging mass spectrometry.

ABSTRACT:

Objective

Glucose is the major energy substrate of the brain and crucial for normal brain function. In diabetes, the brain is subject to episodes of hypo- and hyperglycemia resulting in acute outcomes ranging from confusion to seizures, while chronic metabolic dysregulation puts patients at increased risk for depression and Alzheimer's disease. In the present study, we aimed to determine how glucose is metabolized in different regions of the brain using imaging mass spectrometry (IMS).

Methods

To examine the relative abundance of glucose and other metabolites in the brain, mouse brain sections were subjected to imaging mass spectrometry at a resolution of 100 μm. This was correlated with immunohistochemistry, qPCR, western blotting and enzyme assays of dissected brain region

SUBMITTER: Kleinridders A 

PROVIDER: S-EPMC6001904 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature