MTORC1-dependent increase in oxidative metabolism in POMC neurons regulates food intake and action of leptin.

ABSTRACT:

Objective

Nutrient availability modulates reactive oxygen species (ROS) production in the hypothalamus. In turn, ROS regulate hypothalamic neuronal activity and feeding behavior. The mechanistic target of rapamycin complex 1 (mTORC1) pathway is an important cellular integrator of the action of nutrients and hormones. Here we tested the hypothesis that modulation of mTORC1 activity, particularly in Proopiomelanocortin (POMC)-expressing neurons, mediates the cellular and behavioral effects of ROS.

Methods

C57BL/6J mice or controls and their knockout (KO) littermates deficient either for the mTORC1 downstream target 70-kDa ribosomal protein S6 kinase 1 (S6K1) or for the mTORC1 component Rptor specifically in POMC neurons (POMC-rptor-KO) were treated with an intracerebroventricu

SUBMITTER: Haissaguerre M 

PROVIDER: S-EPMC6001919 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature