Profiling of G protein-coupled receptors in vagal afferents reveals novel gut-to-brain sensing mechanisms.

ABSTRACT:

Objectives

G protein-coupled receptors (GPCRs) act as transmembrane molecular sensors of neurotransmitters, hormones, nutrients, and metabolites. Because unmyelinated vagal afferents richly innervate the gastrointestinal mucosa, gut-derived molecules may directly modulate the activity of vagal afferents through GPCRs. However, the types of GPCRs expressed in vagal afferents are largely unknown. Here, we determined the expression profile of all GPCRs expressed in vagal afferents of the mouse, with a special emphasis on those innervating the gastrointestinal tract.

Methods

Using a combination of high-throughput quantitative PCR, RNA sequencing, and in situ hybridization, we systematically quantified GPCRs expressed in vagal unmyelinated Na_v1.8-expressing afferents.<

SUBMITTER: Egerod KL 

PROVIDER: S-EPMC6001940 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature