Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species.

ABSTRACT: Information about species distribution is crucial to ecological studies. Environmental DNA (eDNA) analysis has recently been used to estimate the distribution of aquatic organisms. Several analytical methods including metabarcoding and species-specific PCR are being used for eDNA analysis. However, when only a few species are targeted, metabarcoding is not cost-effective because of the wasted consumption of read due to amplification of non-target species DNA. On the other hand, species-specific PCR requires tests to be repeated multiple times resulting in consuming more DNA templates, and experimental consumables. Here we propose a methodological framework for simultaneously detecting a few species using real-time multiplex PCR. We developed the species-specific primer-probe sets for two s

SUBMITTER: Tsuji S 

PROVIDER: S-EPMC6002393 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature