Acoustic impedance matched buffers enable separation of bacteria from blood cells at high cell concentrations.

ABSTRACT: Sepsis is a common and often deadly systemic response to an infection, usually caused by bacteria. The gold standard for finding the causing pathogen in a blood sample is blood culture, which may take hours to days. Shortening the time to diagnosis would significantly reduce mortality. To replace the time-consuming blood culture we are developing a method to directly separate bacteria from red and white blood cells to enable faster bacteria identification. The blood cells are moved from the sample flow into a parallel stream using acoustophoresis. Due to their smaller size, the bacteria are not affected by the acoustic field and therefore remain in the blood plasma flow and can be directed to a separate outlet. When optimizing for sample throughput, 1 ml of undiluted whole blood equivalent

SUBMITTER: Ohlsson P 

PROVIDER: S-EPMC6002537 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature