Transcript variants and expression profiles analysis of Mitf gene in minipigs.

ABSTRACT:

Object

To identify transcript variants and expression patterns of porcine Mitf.

Materials and methods

A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues.

Results

Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS.

Conclusion

The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human

SUBMITTER: Guo W 

PROVIDER: S-EPMC6002570 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature