Inflammasome activation in mouse inner ear in response to MCMV induced hearing loss.

ABSTRACT:

Objective

To identify presence of inflammasome activated in mouse cochlea with sensorineural hearing loss (SNHL) caused by cytomegalovirus (CMV) infection.

Method

MCMV was injected into the right cerebral hemisphere in neonatal BALB/c mice at 2000 pfu virus titers. Auditory brainstem responses (ABRs) were tested to evaluate hearing at 21 days. Histopathological studies were conducted to confirm localizations of MCMV infected cells in the inner ear. Expression of inflammasome related factors was assessed by immunofluorescence, Quantitative real-time PCR and Western blotting.

Results

In the mouse model of CMV induced SNHL, inflammasome related kinase Caspase-1 and downstream inflammatory factor IL-1β and IL-18 were found increased and activated after CMV infection in th

SUBMITTER: Shi X 

PROVIDER: S-EPMC6002578 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature