Mass Spectrometry-Based Method to Study Inhibitor-Induced Metabolic Redirection in the Central Metabolism of Cancer Cells.

ABSTRACT: Cancer cells often respond to chemotherapeutic inhibitors by redirecting carbon flow in the central metabolism. To understand the metabolic redirections of inhibitor treatment on cancer cells, this study established a ¹³C-metabolic flux analysis (¹³C-MFA)-based method to evaluate metabolic redirection in MCF-7 breast cancer cells using mass spectrometry. A metabolic stationary state necessary for accurate ¹³C-MFA was confirmed during an 8-24 h window using low-dose treatments of various metabolic inhibitors. Further ¹³C-labeling experiments using [1-¹³C]glucose and [U-¹³C]glutamine, combined with gas chromatography-mass spectrometry (GC-MS) analysis of mass isotopomer distributions (MIDs), confirmed that an isotopic stationa

SUBMITTER: Araki C 

PROVIDER: S-EPMC6002601 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature