Optimizing the method for generation of integration-free induced pluripotent stem cells from human peripheral blood.

ABSTRACT: BACKGROUND:Generation of induced pluripotent stem cells (iPSCs) from human peripheral blood provides a convenient and low-invasive way to obtain patient-specific iPSCs. The episomal vector is one of the best approaches for reprogramming somatic cells to pluripotent status because of its simplicity and affordability. However, the efficiency of episomal vector reprogramming of adult peripheral blood cells is relatively low compared with cord blood and bone marrow cells. METHODS:In the present study, integration-free human iPSCs derived from peripheral blood were established via episomal technology. We optimized mononuclear cell isolation and cultivation, episomal vector promoters, and a combination of transcriptional factors to improve reprogramming efficiency. RESULTS:Here, we improved the

SUBMITTER: Gu H 

PROVIDER: S-EPMC6002980 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature