A pivotal role of BEX1 in liver progenitor cell expansion in mice.

ABSTRACT: BACKGROUND:The activation and expansion of bipotent liver progenitor cells (LPCs) are indispensable for liver regeneration after severe or chronic liver injury. However, the underlying molecular mechanisms regulating LPCs and LPC-mediated liver regeneration remain elusive. METHODS:Hepatic brain-expressed X-linked 1 (BEX1) expression was evaluated using microarray screening, real-time polymerase chain reaction, immunoblotting and immunofluorescence. LPC activation and liver injury were studied following a choline-deficient, ethionine-supplemented (CDE) diet in wild-type (WT) and Bex1-/- mice. Proliferation, apoptosis, colony formation and hepatic differentiation were examined in LPCs from WT and Bex1-/- mice. Peroxisome proliferator-activated receptor gamma was detected in Bex1-deficient LP

SUBMITTER: Gu Y 

PROVIDER: S-EPMC6002993 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature