Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease.

ABSTRACT: BACKGROUND:The ability of circulating monocytes to develop into lung macrophages and promote lung tissue damage depends upon their phenotypic pattern of differentiation and activation. Whether this phenotypic pattern varies with COPD severity is unknown. Here we characterize the activation and differentiation status of circulating monocytes in patients with moderate vs. severe COPD. METHODS:Blood monocytes were isolated from normal non-smokers (14), current smokers (13), patients with moderate (9), and severe COPD (11). These cells were subjected to analysis by flow cytometry to characterize the expression of activation markers, chemoattractant receptors, and surface markers characteristic of either M1- or M2-type macrophages. RESULTS:Patients with severe COPD had increased numbers of tota

SUBMITTER: Cornwell WD 

PROVIDER: S-EPMC6003040 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature