Temporal changes in macrophage phenotype after peripheral nerve injury.

ABSTRACT:

Background

Macrophages play a key role in peripheral nerve repair and demonstrate complex phenotypes that are highly dependent on microenvironmental cues.

Methods

We determined temporal changes in macrophage gene expression over time using RNA sequencing after fluorescence-activated cell sorting (FACS) macrophage populations from injured peripheral nerve. We identified key upstream regulators and dominant pathways using ingenuity pathway analysis and confirmed these changes with NanoString technology. We then investigate the effects of extreme polarizers of macrophage phenotype (IL4 and IFNγ) on nerve regeneration. We determined macrophage gene expression in vivo at the site of peripheral nerve injury with NanoString technology, and assessed recovery from sciatic nerve injur

SUBMITTER: Tomlinson JE 

PROVIDER: S-EPMC6003127 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature