Differential equation methods for simulation of GFP kinetics in non-steady state experiments.

ABSTRACT: Genetically encoded fluorescent proteins, combined with fluorescence microscopy, are widely used in cell biology to collect kinetic data on intracellular trafficking. Methods for extraction of quantitative information from these data are based on the mathematics of diffusion and tracer kinetics. Current methods, although useful and powerful, depend on the assumption that the cellular system being studied is in a steady state, that is, the assumption that all the molecular concentrations and fluxes are constant for the duration of the experiment. Here, we derive new tracer kinetic analytical methods for non-steady state biological systems by constructing mechanistic nonlinear differential equation models of the underlying cell biological processes and linking them to a separate set of diffe

SUBMITTER: Phair RD 

PROVIDER: S-EPMC6003217 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature