Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

ABSTRACT: MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of Mtor in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneration of oocytes and loss of granulosa cell identity coincident with the acquisition of immature Sertoli cell-like characteristics. Although Mtor was deleted at the primordial oocyte stage, DNA damage accumulated in oocytes during their later growth, and there was a marked alteration of the trans

SUBMITTER: Guo J 

PROVIDER: S-EPMC6003357 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature