Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses.

ABSTRACT: Glutamatergic synapses display a rich repertoire of plasticity mechanisms on many different time scales, involving dynamic changes in the efficacy of transmitter release as well as changes in the number and function of postsynaptic glutamate receptors. The genetically encoded glutamate sensor iGluSnFR enables visualization of glutamate release from presynaptic terminals at frequencies up to ∼10 Hz. However, to resolve glutamate dynamics during high-frequency bursts, faster indicators are required. Here, we report the development of fast (iGlu _f ) and ultrafast (iGlu _u ) variants with comparable brightness but increased K_d for glutamate (137 μM and 600 μM, respectively). Compared with iGluSnFR, iGlu _u has a sixfold fast

SUBMITTER: Helassa N 

PROVIDER: S-EPMC6003469 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature