Codon usage of highly expressed genes affects proteome-wide translation efficiency.

ABSTRACT: Although the genetic code is redundant, synonymous codons for the same amino acid are not used with equal frequencies in genomes, a phenomenon termed "codon usage bias." Previous studies have demonstrated that synonymous changes in a coding sequence can exert significant cis effects on the gene's expression level. However, whether the codon composition of a gene can also affect the translation efficiency of other genes has not been thoroughly explored. To study how codon usage bias influences the cellular economy of translation, we massively converted abundant codons to their rare synonymous counterpart in several highly expressed genes in Escherichia coli This perturbation reduces both the cellular fitness and the translation efficiency of genes that have high initiation rat

SUBMITTER: Frumkin I 

PROVIDER: S-EPMC6003480 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature