Simulated night shift work induces circadian misalignment of the human peripheral blood mononuclear cell transcriptome.

ABSTRACT: Misalignment of the endogenous circadian timing system leads to disruption of physiological rhythms and may contribute to the development of the deleterious health effects associated with night shift work. However, the molecular underpinnings remain to be elucidated. Here, we investigated the effect of a 4-day simulated night shift work protocol on the circadian regulation of the human transcriptome. Repeated blood samples were collected over two 24-hour measurement periods from eight healthy subjects under highly controlled laboratory conditions before and 4 days after a 10-hour delay of their habitual sleep period. RNA was extracted from peripheral blood mononuclear cells to obtain transcriptomic data. Cosinor analysis revealed a marked reduction of significantly rhythmic transcripts in

SUBMITTER: Kervezee L 

PROVIDER: S-EPMC6003514 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature