Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors.

ABSTRACT: Cells in tissues undergo transdifferentiation programs when stimulated by specific mechanical and biochemical signals. While seminal studies have demonstrated that exogenous biochemical factors can reprogram somatic cells into pluripotent stem cells, the critical roles played by mechanical signals in such reprogramming process have not been well documented. In this paper, we show that laterally confined growth of fibroblasts on micropatterned substrates induces nuclear reprogramming with high efficiency in the absence of any exogenous reprogramming factors. We provide compelling evidence on the induction of stem cell-like properties using alkaline phosphatase assays and expression of pluripotent markers. Early onset of reprogramming was accompanied with enhanced nuclear dynamics and change

SUBMITTER: Roy B 

PROVIDER: S-EPMC6003522 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature