Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

ABSTRACT: Glycosylation, the covalent attachment of carbohydrate structures onto proteins, is the most abundant post-translational modification. Over 50% of human proteins are glycosylated, which alters their activities in diverse fundamental biological processes. Despite the importance of glycosylation in biology, the identification and functional validation of complex glycoproteins has remained largely unexplored. Here we develop a novel quantitative approach to identify intact glycopeptides from comparative proteomic data sets, allowing us not only to infer complex glycan structures but also to directly map them to sites within the associated proteins at the proteome scale. We apply this method to human and mouse embryonic stem cells to illuminate the stem cell glycoproteome. This analysis nearly

SUBMITTER: Stadlmann J 

PROVIDER: S-EPMC6003595 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature