Simultaneous LC-MS/MS analysis of eicosanoids and related metabolites in human serum, sputum and BALF.

ABSTRACT: The differences among individual eicosanoids in eliciting different physiological and pathological responses are largely unknown because of the lack of valid and simple analytical methods for the quantification of individual eicosanoids and their metabolites in serum, sputum and bronchial alveolar lavage fluid (BALF). Therefore, a simple and sensitive LC-MS/MS method for the simultaneous quantification of 34 eicosanoids in human serum, sputum and BALF was developed and validated. This method is valid and sensitive with a limit of quantification ranging from 0.2 to 3 ng/mL for the various analytes, and has a large dynamic range (500 ng/mL) and a short run time (25 min). The intra- and inter-day accuracy and precision values met the acceptance criteria according to US Food and Drug Administr

SUBMITTER: Thakare R 

PROVIDER: S-EPMC6003856 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature