LlamaTags: A Versatile Tool to Image Transcription Factor Dynamics in Live Embryos.

ABSTRACT: Embryonic cell fates are defined by transcription factors that are rapidly deployed, yet attempts to visualize these factors in vivo often fail because of slow fluorescent protein maturation. Here, we pioneer a protein tag, LlamaTag, which circumvents this maturation limit by binding mature fluorescent proteins, making it possible to visualize transcription factor concentration dynamics in live embryos. Implementing this approach in the fruit fly Drosophila melanogaster, we discovered stochastic bursts in the concentration of transcription factors that are correlated with bursts in transcription. We further used LlamaTags to show that the concentration of protein in a given nucleus heavily depends on transcription of that gene in neighboring nuclei; we speculate that this inter-nuclear sig

SUBMITTER: Bothma JP 

PROVIDER: S-EPMC6003873 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature