MMP-3 in the peripheral serum as a biomarker of knee osteoarthritis, 40 years after open total knee meniscectomy.

ABSTRACT:

Background

To explore potential biomarkers in a meniscectomy-induced knee osteoarthritis model, at forty years after meniscectomy.

Methods

We carried out a forty-year study of 53 patients who, as adolescents, underwent open total meniscectomy and assessed two potential synovial and serum biomarkers, namely glycosaminoglycan (GAG) and matrix metalloproteinase-3 (MMP-3). Of the 30 patients available for review, 8 had contralateral knee operations and were excluded. Of the remaining 22 patients, 17 had successful operated knee synovial fluid aspirations and 8 also had successful contralateral control knee aspirations. GAG and MMP3 levels in the synovial fluid and peripheral serum was measured using Alcian blue precipitation and ELISA quantification, respectively. Patients also

SUBMITTER: Pengas I 

PROVIDER: S-EPMC6003895 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature