Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.

ABSTRACT:

Background

α-D-Glucosylglycerol (αGG) has beneficial functions as a moisturizing agent in cosmetics and potential as a health food material, and therapeutic agent. αGG serves as compatible solute in various halotolerant cyanobacteria such as Synechocystis sp. PCC 6803, which synthesizes αGG in a two-step reaction: The enzymatic condensation of ADP-glucose and glycerol 3-phosphate by GG-phosphate synthase (GGPS) is followed by the dephosphorylation of the intermediate by the GG-phosphate phosphatase (GGPP). The Gram-positive Corynebacterium glutamicum, an industrial workhorse for amino acid production, does not utilize αGG as a substrate and was therefore chosen for the development of a heterologous microbial production platform for αGG.

Results

Plasmid-bound expression of gg

SUBMITTER: Roenneke B 

PROVIDER: S-EPMC6004087 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature