Tackling critical parameters in metazoan meta-barcoding experiments: a preliminary study based on coxI DNA barcode.

ABSTRACT: Nowadays DNA meta-barcoding is a powerful instrument capable of quickly discovering the biodiversity of an environmental sample by integrating the DNA barcoding approach with High Throughput Sequencing technologies. It mainly consists of the parallel reading of informative genomic fragment/s able to discriminate living entities. Although this approach has been widely studied, it still needs optimization in some necessary steps requested in its advanced accomplishment. A fundamental element concerns the standardization of bioinformatic analyses pipelines. The aim of the present study was to underline a number of critical parameters of laboratory material preparation and taxonomic assignment pipelines in DNA meta-barcoding experiments using the cytochrome oxidase subunit-I (coxI) barc

SUBMITTER: Balech B 

PROVIDER: S-EPMC6004112 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature