Tandem Mass Spectrometry and Ion Mobility Reveals Structural Insight into Eicosanoid Product Ion Formation.

ABSTRACT: Ion mobility measurements of product ions were used to characterize the collisional cross section (CCS) of various complex lipid [M-H]^- ions using traveling wave ion mobility mass spectrometry (TWIMS). TWIMS analysis of various product ions derived after collisional activation of mono- and dihydroxy arachidonate metabolites was found to be more complex than the analysis of intact molecular ions and provided some insight into molecular mechanisms involved in product ion formation. The CCS observed for the molecular ion [M-H]^- and certain product ions were consistent with a folded ion structure, the latter predicted by the proposed mechanisms of product ion formation. Unexpectedly, product ions from [M-H-H₂O-CO₂]^- and [M-H-H₂O

SUBMITTER: Di Giovanni JP 

PROVIDER: S-EPMC6004252 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature