Ectonucleotidase-Mediated Suppression of Lupus Autoimmunity and Vascular Dysfunction.

ABSTRACT:

Objectives

CD39 and CD73 are surface enzymes that jut into the extracellular space where they mediate the step-wise phosphohydrolysis of the autocrine and paracrine danger signals ATP and ADP into anti-inflammatory adenosine. Given the role of vascular and immune cells' "purinergic halo" in maintaining homeostasis, we hypothesized that the ectonucleotidases CD39 and CD73 might play a protective role in lupus.

Methods

Lupus was modeled by intraperitoneal administration of pristane to three groups of mice: wild-type (WT), CD39^-/-, and CD73^-/-. After 36 weeks, autoantibodies, endothelial function, kidney disease, splenocyte activation/polarization, and neutrophil activation were characterized.

Results

As compared with WT mice, CD39^-/- mic

SUBMITTER: Knight JS 

PROVIDER: S-EPMC6004379 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature