MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution.

ABSTRACT: Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by cent

SUBMITTER: Eilers Y 

PROVIDER: S-EPMC6004438 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature