Metaproteomics method to determine carbon sources and assimilation pathways of species in microbial communities.

ABSTRACT: Measurements of stable carbon isotope ratios (δ¹³C) are widely used in biology to address questions regarding food sources and metabolic pathways used by organisms. The analysis of these so-called stable isotope fingerprints (SIFs) for microbes involved in biogeochemical cycling and microbiota of plants and animals has led to major discoveries in environmental microbiology. Currently, obtaining SIFs for microbial communities is challenging as the available methods either only provide low taxonomic resolution, such as the use of lipid biomarkers, or are limited in throughput, such as nanoscale secondary ion MS imaging of single cells. Here we present "direct protein-SIF" and the Calis-p software package (https://sourceforge.net/projects/calis-p/), which enable high-throughput mea

SUBMITTER: Kleiner M 

PROVIDER: S-EPMC6004456 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature