Transcriptional Regulation of Cysteine and Methionine Metabolism in Lactobacillus paracasei FAM18149.

ABSTRACT: Lactobacillus paracasei is common in the non-starter lactic acid bacteria (LAB) community of raw milk cheeses. This species can significantly contribute to flavor formation through amino acid metabolism. In this study, the DNA and RNA of L. paracasei FAM18149 were sequenced using next-generation sequencing technologies to reconstruct the metabolism of the sulfur-containing amino acids cysteine and methionine. Twenty-three genes were found to be involved in cysteine biosynthesis, the conversion of cysteine to methionine and vice versa, the S-adenosylmethionine recycling pathway, and the transport of sulfur-containing amino acids. Additionally, six methionine-specific T-boxes and one cysteine-specific T-box were found. Five of these were located upstream of genes encodin

SUBMITTER: Wuthrich D 

PROVIDER: S-EPMC6004538 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature