Trajectories of cell-cycle progression from fixed cell populations.

ABSTRACT: An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.

SUBMITTER: Gut G 

PROVIDER: S-EPMC6004611 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature