Genomic Sequencing of Bordetella pertussis for Epidemiology and Global Surveillance of Whooping Cough.

ABSTRACT: Bordetella pertussis causes whooping cough, a highly contagious respiratory disease that is reemerging in many world regions. The spread of antigen-deficient strains may threaten acellular vaccine efficacy. Dynamics of strain transmission are poorly defined because of shortcomings in current strain genotyping methods. Our objective was to develop a whole-genome genotyping strategy with sufficient resolution for local epidemiologic questions and sufficient reproducibility to enable international comparisons of clinical isolates. We defined a core genome multilocus sequence typing scheme comprising 2,038 loci and demonstrated its congruence with whole-genome single-nucleotide polymorphism variation. Most cases of intrafamilial groups of isolates or of multiple isolates recovered from the sam

SUBMITTER: Bouchez V 

PROVIDER: S-EPMC6004856 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature