Mobile colistin resistance gene mcr-5 in porcine Aeromonas hydrophila.

ABSTRACT:

Objectives

To characterize the mobile colistin resistance gene mcr-5 in Aeromonas hydrophila from backyard pigs in rural areas of China.

Methods

Pig faecal samples from 194 households were directly tested for the presence of mcr-5 by PCR assay and the phenotypic antimicrobial susceptibility profiles of the mcr-5-positive isolates were determined using the broth dilution method. The genomic location and transferability of mcr-5 were analysed by S1-PFGE with Southern blotting and DNA hybridization, and natural transformation, respectively. One strain isolated from an mcr-5-positive sample was subjected to WGS and the stability of the mcr-5-harbouring plasmid over successive generations was examined by subculturing.

Results

One mcr-5-positive A. hydrophila isolate showin

SUBMITTER: Ma S 

PROVIDER: S-EPMC6005042 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature