Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions.

ABSTRACT: Diseases are characterized by distinct changes in tissue molecular distribution. Molecular analysis of intact tissues traditionally requires preexisting knowledge of, and reagents for, the targets of interest. Conversely, label-free discovery of disease-associated tissue analytes requires destructive processing for downstream identification platforms. Tissue-based analyses therefore sacrifice discovery to gain spatial distribution of known targets or sacrifice tissue architecture for discovery of unknown targets. To overcome these obstacles, we developed a multimodality imaging platform for discovery-based molecular histology. We apply this platform to a model of disseminated infection triggered by the pathogen Staphylococcus aureus, leading to the discovery of infection-associated

SUBMITTER: Cassat JE 

PROVIDER: S-EPMC6005374 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature