Long-read whole genome sequencing and comparative analysis of six strains of the human pathogen Orientia tsutsugamushi.

ABSTRACT:

Background

Orientia tsutsugamushi is a clinically important but neglected obligate intracellular bacterial pathogen of the Rickettsiaceae family that causes the potentially life-threatening human disease scrub typhus. In contrast to the genome reduction seen in many obligate intracellular bacteria, early genetic studies of Orientia have revealed one of the most repetitive bacterial genomes sequenced to date. The dramatic expansion of mobile elements has hampered efforts to generate complete genome sequences using short read sequencing methodologies, and consequently there have been few studies of the comparative genomics of this neglected species.

Results

We report new high-quality genomes of O. tsutsugamushi, generated using PacBio single molecule long read sequencing, for

SUBMITTER: Batty EM 

PROVIDER: S-EPMC6005640 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature