Cell Specific Changes of Autophagy in a Mouse Model of Contusive Spinal Cord Injury.

ABSTRACT: Autophagy is an essential process of cellular waist clearance that becomes altered following spinal cord injury (SCI). Details on these changes, including timing after injury, underlying mechanisms, and affected cells, remain controversial. Here we present a characterization of autophagy in the mice spinal cord before and after a contusive SCI. In the undamaged spinal cord, analysis of LC3 and Beclin 1 autophagic markers reveals important differences in basal autophagy between neurons, oligodendrocytes, and astrocytes and even within cell populations. Following moderate contusion, western blot analyses of LC3 indicates that autophagy increases to a maximum at 7 days post injury (dpi), whereas unaltered Beclin 1 expression and increase of p62 suggests a possible blockage of autophagosome cl

SUBMITTER: Munoz-Galdeano T 

PROVIDER: S-EPMC6005838 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature