The Complexome of Dehalococcoides mccartyi Reveals Its Organohalide Respiration-Complex Is Modular.

ABSTRACT: Dehalococcoides mccartyi strain CBDB1 is a slow growing strictly anaerobic microorganism dependent on halogenated compounds as terminal electron acceptor for anaerobic respiration. Indications have been described that the membrane-bound proteinaceous organohalide respiration complex of strain CBDB1 is functional without quinone-mediated electron transfer. We here study this multi-subunit protein complex in depth in regard to participating protein subunits and interactions between the subunits using blue native gel electrophoresis coupled to mass spectrometric label-free protein quantification. Applying three different solubilization modes to detach the respiration complex from the membrane we describe different solubilization snapshots of the organohalide respiration complex. The re

SUBMITTER: Seidel K 

PROVIDER: S-EPMC6005880 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature