MHC class I diversity of olive baboons (Papio anubis) unravelled by next-generation sequencing.

ABSTRACT: The olive baboon represents an important model system to study various aspects of human biology and health, including the origin and diversity of the major histocompatibility complex. After screening of a group of related animals for polymorphisms associated with a well-defined microsatellite marker, subsequent MHC class I typing of a selected population of 24 animals was performed on two distinct next-generation sequencing (NGS) platforms. A substantial number of 21 A and 80 B transcripts were discovered, about half of which had not been previously reported. Per animal, from one to four highly transcribed A alleles (majors) were observed, in addition to ones characterised by low transcripion levels (minors), such as members of the A*14 lineage. Furthermore, in one animal, up to 13 B allel

SUBMITTER: van der Wiel MKH 

PROVIDER: S-EPMC6006219 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature