In vitro inhibition of hepatic stellate cell activation by the autophagy-related lipid droplet protein ATG2A.

ABSTRACT: Clinical studies have found that moderate intake of retinol or oleic acid can enlarge the lipid droplets of hepatic stellate cells and suppress their activation. However, the link between lipid droplets and cell activation is unknown. This study compared the dynamics of lipid droplet-associated protein expression between activated and reverted stellate cells. Reversion of the activated human stellate cell line LX-2 and inhibition of primary mouse stellate cell activation were induced by retinol or oleic acid, which resulted in larger lipid droplets and the downregulation of cell activation markers. Quantitative proteomics and immunoblotting were performed to compare lipid-droplet protein profiles between activated and reverted LX-2 cells. Compared to expression in activated cells, 50 lipid

SUBMITTER: Hong Y 

PROVIDER: S-EPMC6006255 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature