MS analysis of a dilution series of bacteria:phytoplankton to improve detection of low abundance bacterial peptides.

ABSTRACT: Assigning links between microbial activity and biogeochemical cycles in the ocean is a primary objective for ecologists and oceanographers. Bacteria represent a small ecosystem component by mass, but act as the nexus for both nutrient transformation and organic matter recycling. There are limited methods to explore the full suite of active bacterial proteins largely responsible for degradation. Mass spectrometry (MS)-based proteomics now has the potential to document bacterial physiology within these complex systems. Global proteome profiling using MS, known as data dependent acquisition (DDA), is limited by the stochastic nature of ion selection, decreasing the detection of low abundance peptides. The suitability of MS-based proteomics methods in revealing bacterial signatures outnumbered

SUBMITTER: Timmins-Schiffman E 

PROVIDER: S-EPMC6006377 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature