RHD-Positive Alleles among D- C/E+ Individuals from India.

ABSTRACT:

Background

Molecular bases of blood group systems, including Rh blood group, have been poorly studied in the Indian population so far, while specificities of Europeans, East Asians and Africans have been well known for years. In order to gain insights into the molecular bases of this population, we sought to characterize the RHD allele in D- Indian donors expressing C and/or E antigen(s).

Methods

RHD gene was analyzed in 171 serologically D-, C/E+ samples by standard molecular methods such as quantitative, multiplex PCR of short fluorescent fragments (QMPSF) and direct sequencing when necessary.

Results

RHD whole gene deletion at the homozygous state was found to be the most common genotype associated with D- phenotype (118/171, 69.0%). Nonfunctio

SUBMITTER: Kulkarni SS 

PROVIDER: S-EPMC6006600 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature